human probdnf (Alomone Labs)
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90
Structured Review
Alomone Labs
human probdnf
Human Probdnf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human probdnf/product/Alomone Labs
Average 90 stars, based on 5 article reviews
Human Probdnf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human probdnf/product/Alomone Labs
Average 90 stars, based on 5 article reviews
human probdnf - by Bioz Stars,
2026-02
90/100 stars
Images
1) Product Images from "N -Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures"
Article Title: N -Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures
Journal: The Journal of Biological Chemistry
doi: 10.1074/jbc.RA119.009989
Figure Legend Snippet: ProBDNF stably expressed in HEK293F cells is glycosylated. N-Glycosylation of proBDNF and the prodomain is documented by mass shift following deglycosylation with PNGase F: A, detection of proBDNF and BDNF by an antibody recognizing the BDNF region; B, detection of proBDNF and the prodomain by an antibody recognizing the prodomain region. The gel images were spliced as indicated by space to exclude samples not related to the study.
Techniques Used: Stable Transfection
![Extracted ion chromatograms (XIC) of (A) nonglycosylated, NYLDAANMSMR and (B) deglycosylated, NYLDAADMSMR glycopeptide of proBDNF secreted by HEK293F cells confirm site occupancy higher than 99%. C, tandem mass spectrum verifies deglycosylation of the proBDNF peptide under [18O]water.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_1326/pmc06851326/pmc06851326__zbc9991914080002.jpg "... nonglycosylated, NYLDAANMSMR and (B) deglycosylated, NYLDAADMSMR glycopeptide of proBDNF secreted by HEK293F cells confirm site occupancy higher ...")
Figure Legend Snippet: Extracted ion chromatograms (XIC) of (A) nonglycosylated, NYLDAANMSMR and (B) deglycosylated, NYLDAADMSMR glycopeptide of proBDNF secreted by HEK293F cells confirm site occupancy higher than 99%. C, tandem mass spectrum verifies deglycosylation of the proBDNF peptide under [18O]water.
Techniques Used:
Figure Legend Snippet: Expression of wildtype (WT) proBDNF and its N121Q mutant (NQ) in stably transfected HEK293F cells. Protein expression was determined by Western blotting using an antibody, recognizing both BDNF and proBDNF, in the conditioned media (A) and cell lysates (B). Arrows point to (pro)BDNF forms: glycosylated proBDNF (black arrow), nonglycosylated proBDNF (white arrow), and mature BDNF (gray arrow). M indicates MagicMark molecular weight marker. Expression of mRNA was determined in WT and N121Q mutant by RT-PCR (C). ProBDNF/BDNF expression in cell lysates (D) or conditioned media (E) of HEK293F cells expressing N121Q mutant treated with proteasome inhibitor MG132 (MG), lysosomal inhibitor chloroquine (CQ), or chemical chaperone 4-phenylbutyric acid (PBA). The black arrow points to the position of nonglycosylated proBDNF.
Techniques Used: Expressing, Mutagenesis, Stable Transfection, Transfection, Western Blot, Molecular Weight, Marker, Reverse Transcription Polymerase Chain Reaction
Figure Legend Snippet: Kinetics of cleavage of glycosylated and nonglycosylated proBDNF by furin. Mass difference between glycosylated and nonglycosylated proBDNF enabled densitometric quantification of each proBDNF form: A, representative blot of the cleavage kinetic at the indicated time intervals; B, densitometric analysis of proBDNF intensities at the indicated time intervals. Results are expressed as percent of the proBDNF concentration (mean ± S.D., n = 3) at the beginning of the reaction. Note that proBDNF without C-terminal Myc-FLAG tag was used in the reaction as described under “Experimental procedures.”
Techniques Used: Concentration Assay, FLAG-tag
Figure Legend Snippet: Mass spectra of proBDNF under the following conditions. A, precursor profile of the tryptic glycopeptide NYLDAANM(ox)SM(ox)R of proBDNF expressed in HEK293F cells; B, HCD fragmentation spectrum of the major glycoform (m/z 1132.42) at low collision energy (10% NCE) showing that characteristic LacdiNAc oxonium ion HexNAc-HexNAc (m/z 407.17) and its fucosylated form (m/z 553.22) with complementary y-ions (m/z 1373, 1381, 1454, and 1555) dominate the composition.
Techniques Used:
Figure Legend Snippet: Relative abundance of the glycoforms of HEK293F-produced proBDNF and prodomain expressed as % of all identified glycoforms
Techniques Used: Produced
Figure Legend Snippet: Impact of modulators of glycosylation on the BDNF/proBDNF ratio in HEK293F cells. Western blotting using antibodies to prodomain and mature BDNF regions was analyzed by densitometry for the following: A, intracellular BDNF to proBDNF ratio; B, secreted BDNF to proBDNF ratio; C, intracellular prodomain to proBDNF ratio; and D, secreted prodomain to proBDNF ratio. The representative Western blotting is labeled as the graphs: CTRL, control; CHLOR, 50 mm sodium chlorate; KIF, 1 μg/ml kifunensine; TNMC, 5 μg/ml of tunicamycin. Results are expressed as scatter plot (mean ± S.D., n = 3). *, p < 0.05; **, p < 0.01; ***, p < 0.001 compared with CTRL, one-way analysis of variance with Bonferroni adjustment.
Techniques Used: Western Blot, Labeling
